Answer;
1. Hydrogen ions cannot freely pass through the inner mitochondrial membrane.
2. H+ concentration is lower in the mitochondrial matrix than in the intermembrane space.
3. H+ ions move through a channel formed by ATP synthase releasing energy to form ATP.
Explanation;
- The electron transport chain forms a proton gradient across the inner mitochondrial membrane, which drives the synthesis of ATP via chemiosmosis.
- Energy released in electron transport reactions is captured as a proton gradient, which is then used to make ATP in a process called chemiosmosis.
- The electron transport chain and chemiosmosis make up oxidative phosphorylation.